- Product nameAnti-Bad抗体[Y208] (DyLight? 488)
参阅全部 Bad 一抗 - Description
兔单克隆抗体[Y208] to Bad (DyLight? 488) - ConjugationDyLight? 488. Ex: 493nm, Em: 518nm
- Specificityab139836 does not cross-react with other Bcl-2 members.
- Tested applicationsFlow Cytmore details
- Species reactivity
Reacts with: Mouse, Rat, Human - Immunogen
Synthetic peptide corresponding to residues at the N terminal of Human Bad (UniProt ID: Q92934). - Positive control
- General notesProduced under U.S. Patent No. 5,675,063.
Properties Applications Our Abpromise guarantee covers the use of ab139836 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application | Abreviews | Notes |
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Flow Cyt |
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Target - FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
- Tissue specificityExpressed in a wide variety of tissues.
- Sequence similaritiesBelongs to the Bcl-2 family.
- DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
- Post-translational
modificationsPhosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. - Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
- Information by UniProt
Database links - Entrez Gene: 572 Human
- Entrez Gene: 12015 Mouse
- Entrez Gene: 64639 Rat
- Omim: 603167 Human
- SwissProt: Q92934 Human
- SwissProt: Q61337 Mouse
- SwissProt: O35147 Rat
- Unigene: 370254 Human
see all Alternative names - BAD antibody
- BAD_HUMAN antibody
- BBC 2 antibody
see all
Anti-Bad antibody [Y208] (DyLight? 488) images Flow Cytometry - Anti-Bad antibody [Y208] (DyLight? 488) - N-terminal (ab139836) Flow cytometric analysis of HeLa cells labelling Bad with ab139836 at 1/50 dilution (red) compared to a negative control antibody (green).
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