18021003406
18021003406
点击这里给我发消息
Elisa试剂盒
标准品/对照品
抗体
血清
国产培养基
生物试剂
进口品牌
耗材
蛋白/多肽
测试盒
细胞
培养基
分子生物学试剂
蛇毒试剂
化学试剂
invitrogen
abcam抗体
beckman coulter

地 址:上海闵行区景联路439号,4幢A区410室
电 话:18021003406
手 机:18021003406
传 真:
联系人:王羽
邮 编:201108
网 址:www.shbybio.com
E-mail:1914109725@qq.com

 
 
Anti-Occludin 抗体abcam *(货号ab31721)新年特价5折促销
点击放大
产品型号:
产品名称:
Anti-Occludin 抗体abcam *(货号ab31721)新年特价5折促销
产品报价:
产品特点:
Anti-Occludin抗体(ab31721)
  Anti-Occludin 抗体abcam *(货号ab31721)新年特价5折促销的详细资料:

  • Product nameAnti-Occludin抗体
    参阅全部 Occludin 一抗
  • Description

    兔多克隆抗体to Occludin

  • SpecificityThe immunogen used to raise this antibody has 78% homology with the Mouse Occludin protein. Some customers have successfully used ab31721 with Mouse lysates/tissue. Please contact Abcam Scientific Support for more information.
  • Tested applicationsIHC-FoFr, WB, In-Cell ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Pig
    Predicted to work with: Dog

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Occludin.

    参阅Abcam的专有抗源政策


    (Peptide available as ab34440.)

  • Positive control
    • ab31721 gave a positive signal in Colon (Human), Liver (Human), and Kidney (Human) Tissue Lysates.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration100 ?g at 1 mg/ml 

  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research Areas
    • Signal Transduction

    • Cytoskeleton / ECM

    • Cell Adhesion

    • Tight Junctions

Applications

Our Abpromise guarantee covers the use of ab31721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationAbreviewsNotes
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20978075
WB
Use a concentration of 1 ?g/ml. Detects a band of approximay 63 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Occludin peptide (ab34440).

Abcam recommends using 3% milk as the blocking agent.

In-Cell ELISA
Use at an assay dependent concentration. PubMed: 22689949
ICC/IF
Use a concentration of 1 - 2 ?g/ml.
IHC-P
1/100. PubMed: 19828778

Target

  • FunctionMay play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • Tissue specificityLocalized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
  • Involvement in diseaseDefects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.
  • Sequence similaritiesBelongs to the ELL/occludin family.
    Contains 1 MARVEL domain.
  • DomainThe C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1.
  • Cellular localizationMembrane. Cell junction > tight junction.
  • Information by UniProt
  • Database links

    • Entrez Gene: 403844 Dog
    • Entrez Gene: 100506658 Human
    • Entrez Gene: 397236 Pig
    • Entrez Gene: 83497 Rat
    • Omim: 602876 Human
    • SwissProt: Q28269 Dog
    • SwissProt: Q16625 Human
    • SwissProt: Q6P6T5 Rat

    see all

  • Alternative names

    • BLCPMG antibody
    • FLJ08163 antibody
    • FLJ18079 antibody

    see all

Anti-Occludin antibody images

  • Western blot - Anti-Occludin antibody (ab31721)

    All lanes : Anti-Occludin antibody (ab31721) at 1 ?g/ml

    Lane 1 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
    Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)

    Lysates/proteins at 10 ?g per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 240 kDa,32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab31721 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunocytochemistry/ Immunofluorescence - Occludin antibody (ab31721)

    ICC/IF image of ab31721 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31721, 5?g/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43?M.

  • Western blot - Anti-Occludin antibody (ab31721)

    Anti-Occludin antibody (ab31721) at 1 ?g/ml (blocked with 3% milk) + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 ?g

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-Occludin antibody (ab31721)

This product has been referenced in:

  • Chung CY  et al. Progressive Proximal-to-Distal Reduction in Expression of the Tight Junction Complex in Colonic Epithelium of Virally-Suppressed HIV+ Individuals. PLoS Pathog 10:e1004198 (2014). WB ; Human .Read more (PubMed: 24968145) ?
  • Sumigray K  et al. Cell-cell adhesions and cell contractility are upregulated upon desmosome disruption. PLoS One 9:e101824 (2014). ICC/IF ; Mouse . Read more (PubMed: 25006807) ?

产品相关关键字: Occludin 进口抗体
 如果你对Anti-Occludin 抗体abcam *(货号ab31721)新年特价5折促销感兴趣,想了解更详细的产品信息,填写下表直接与厂家联系:

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
 相关同类产品:
Anti-Bax抗体6A7 abcam进口(ab5714)新年5折促销  Anti-Bad DyLight 488标记抗体abcam(货号ab139836)5折促销  流式标记Anti-Bcl2抗体,abcam原装现货  流式标记Anti-Bad抗体,abcam原装现货 
ab15116Rabbit polyclonal to Aquaporin 2  ab15115Rabbit polyclonal to Oct2 prediluted  ab15114Rabbit polyclonal to Oct2  ab15113Rabbit polyclonal to Oct1 prediluted 
ab15112Rabbit polyclonal to Oct1  ab15111Mouse monoclonal [SPM133] to Fascin prediluted 
 
 
·大鼠绒毛膜促性腺激素β肽(CGβ)Elisa试剂盒
·猪瘟病毒抗体(CSFV Ab)定量检测试剂盒
·ELISA试剂盒的特点和实验规则
·胰高血糖素样肽(GLP-1) 7-36
·拟南芥-IKI3多抗―植物学研究的重要工具
·免疫电镜胶体金标记方法及操做(仅供参考)
·磷酸化抗体产品及制备服务
·干细胞培育出视网膜前体
·穆斯塔夫·巴拉特谈生物乙醇的是与非
·雌激素可抑制病毒感染
上海博耀生物科技有限公司 Copyright(C) 2008 电话:18021003406 传 真:
技术支持:智能制造网 GoogleSiteMap 网站管理入口 邮箱登陆