N-2添加剂是化学成分确定的，不含血清的，基于Bottenstein’s 的N1配方的添加剂。N－2添加剂主要用于Neurobasal®培养基。
它主要被推荐用于成神经细胞瘤以及周围神经系统（PNS）与中枢神经系统（CNS）的原代细胞培养中有丝分裂后期神经元的生长和表达。N－2添加剂可以替代Bottenstein’s的N1添加剂。我们提供的N－2添加剂是100倍的浓缩体。它可用于添加了生长因子bFGF及EGF的Neurobasal®培养基，或DMEM培养基。
N-2 Supplement （100X） liquidFor the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation （1）. This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system （PNS） and the central nervous system （CNS）. It can be used as a substitute for the N-1 Bottenstein formulation. N-2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines. （in Current Methods in Cellular *部分 invitrogen 原装产品 | 品牌 | 货号 | 名称 | 规格 | | invitrogen | 13778-075 | Lipofectamine RNAiMAX | 0.75ml | | invitrogen | 13778-150 | Lipofectamine RNAiMAX | 1.5ml | | invitrogen | 11668-027 | Lipofectamine 2000 Reagent | 0.75ml | | invitrogen | 11668-019 | Lipofectamine 2000 Reagent | 1.5ml | | invitrogen | 15596-026 | TRlzol ®Reagent | 100ml | | invitrogen | 15596-018 | TRlzol ®Reagent | 200ml | | invitrogen | 10296-010 | Trizol ® LS Reagent | 100ml | | invitrogen | 10296-028 | Trizol ® LS Reagent | 200ml | | invitrogen | 17504-044 | B-27（黄颜色） | 10ml | | invitrogen | 12587-010 | B-27（黄颜色）不含维生素A | 10ml | | invitrogen | 17502-048 | N-2 （粉红颜色） | 5ml | | invitrogen | 18064-014 | SuperScript® II Reverse Transcriptase | 10,000U | | invitrogen | 18080-044 | SuperScript® III Reverse Transcriptase | 10,000U | 第二部分 HyClone胎牛血清 | 品牌 | 货号 | 名称 | 规格 | | HyClone | SH30370.03 | Australian Standard Fetal Bovine Serum | 500ml | | HyClone | SH30406.02 | FOETAL BOVINE SERUM | 500ml | | HyClone | SH30401.01 | New Zealand Calf Serum | 500ml | | HyClone | SH30066.02 | FetalClone® II | 100ml | | HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml | | HyClone | SH30118.02 | Newborn Bovine Calf Serum, U.S. Origin | 100ml | | HyClone | SH30070.02 | FETAL BOVINE SERUM | 100ml | | HyClone | SH30087.02 | COSMIC CALF® SERUM | 100ml | | HyClone | SH30070.03E | FETALBOVINESERUM CHARACTERIZSED | 500ml | | 100ml | | HyClone | SH30070.03 | FETAL BOVINE SERUM | 500ml | | HyClone | SH30080.03 | FetalClone® I | 500ml | | HyClone | SH30084.03 | FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN | 500ml | | HyClone | SH30396.03 | FETAL BOVINE SERUM CHARACTERIZED | 500ml | | HyClone | SV30087.02 | 南美源胎牛血清 | 500ml | | HyClone | sv30087.01 | 南美源胎牛血清 | 100ml | | HyClone | SH30071.03 | FETAL BOVINE SERUM | 500ml | | HyClone | SH30071.02 | FETAL BOVINE SERUM | 100ml | | HyClone | SH30080.02 | FetalClone® I | 100ml | | HyClone | SH30070.02E | FETAL BOVINE SERUM CHARACTERIZSED | 100ml | | HyClone | SH30079.02 | FETAL BOVINE SERUM | 100ml | | HyClone | SH30068.02 | CHARCOAL/DEXTRAN TREATED FBS，
活性炭灭活血清 | 100ml | | HyClone | SH30068.03 | CHARCOAL/DEXTRAN TREATED FBS，
活性炭灭活血清 | 500ml | | HyClone | SH30066.03 | FetalClone® II | 500ml | | HyClone | SH30109.03 | HyClone FetalClone® III | 500ml | | HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml | | HyClone | SH30088.02 | STANDARD FETAL BOVINE SERUM | 100ml | | HyClone | SH30079.03 | FETAL BOVINE SERUM | 500ml | 第二部分 Gibco胎牛血清 | 品牌 | 货号 | 名称 | 规格 | | Gibco | 12662-029 | MSC QUALIFIED FBS 500ML | 500ML | | Gibco | 10438-026 | FBS, HI 500ML | 500ML | | Gibco | 10439-024 | FBS, ES CELL QUAL. USDA APPD. 500ML | 500ML | | Gibco | 10099-141 | FBS, AUSTRALIAN ORIGIN 500ML | 500ML | | Gibco | 26400-044 | FBS, DIALYZED, USA ORIGIN 500ML | 500ML | | Gibco | 26140-079 | FBS QUALIFIED, USA ORIGIN 500ML | 500ML | | Gibco | 16141-079 | FBS, ES CELL QUAL, USA ORIGIN 500ML | 500ML | | Gibco | 16140-071 | FBS, HI 500ML | 500ML | | Gibco | 16000-044 | FBS, CERT, USA ORIGIN 500ML | 500ML | | Gibco | C2027050 | FBS, URUGUAYAN ORIGIN | 500ML | | Gibco | 12657-029 | 巴西胎牛 | 500ml | |
